ABSTRACT
Aim To evaluate the improvement of glycemic control and stress adaptation in patients with GDM by mobile phone WeChat management during novel coronavirus pneumonia. Methods In this study, 75 women with GDM were included, of whom 35 were included in mobile WeChat group management as the GDM-M group and 40 as the GDM group. Results After mobile WeChat group management for 4 weeks, E and NE were lower. MDA was lower, and SOD was higher. HOMA-IR was lower. E, NE, and cortisol were related to HOMA-IR positively, MDA was positively related to HOMA-IR, and SOD was negatively related to HOMA-IR. E and cortisol were positively related to MDA but negatively related to SOD. Conclusion The stress adaptation disorder and insulin resistance in patients with GDM who have completed mobile WeChat group management can be improved during novel coronavirus pneumonia. Mobile WeChat management played a positive role in improving the insulin resistance of women with GDM under special circumstances, which may reduce the risk of maternal and fetal complications.
ABSTRACT
Real-time fluorescent quantitative PCR is considered as the gold standard for diagnosing COVID-19 worldwide now. But RT-PCR which could yield semi-quantitative results only, and the sensitivity of this method is usually not sufficient to discriminate patients in early stages of infection or with a very low viral load. So, the false-negative phenomenon often brings difficulties to epidemic prevention and diagnosis. More accurate and robust determine method are required for accurate SARS-CoV-2 diagnosis. To offer an attractive platform for SARS-CoV-2 RNA quantification, we report a droplet digital PCR technique for efficient, accurate, and quantitative detection of SARS-CoV-2 RNA. Two pairs of primer and two double-quenched probes targeting the region of ORF1ab and N protein coding genes of SARS-CoV-2 were designed to develop ddPCR assay. The sensitivity, specificity, repeatability, and reproducibility were tested by standard cDNAs and clinical specimens. First, we found the optimal annealing temperature was 53.6℃, the optimal primer concentration was 500 nM per reaction. When both probes of ORF1ab and N protein are applied simultaneously, their concentrations are100 nM and 50 nM, respectively. The optimal concentration of SARS-CoV-2 standard cDNA ranged from 1×103 to 1×104 copies/µL. And under this condition, the trend line for the measured concentration is Y = 0.9978x-0.4435. Further, the ddPCR were approximately 10 folds greater sensitivity than RT-qPCR assay. The established ddPCR assay with both probes showed to be a highly effective, accurate and reliable method for the sensitive detection of SARS-CoV-2. And the ddPCR method is the most potential and valuable method for clinical detection of SARS-CoV-2 virus.
Subject(s)
COVID-19ABSTRACT
Objective: To explore the clinical characteristics and risk factors of mortality in the elderly patients with novel coronavirus pneumonia(COVID-19).
ABSTRACT
Background: While pre-existing cardiovascular disease (CVD) appears to be associated with poor outcomes in patients with Coronavirus Disease 2019 (COVID-19), data on patients with CVD and concomitant cancer is limited. Evaluate the effect of underlying CVD and CVD risk factors with cancer history on in-hospital mortality in those with COVID-19.Methods: Data from symptomatic adults hospitalized with COVID-19 at 86 hospitals in the US enrolled in the American Heart Association’s COVID-19 CVD Registry was analyzed. The primary exposure was cancer history. The primary outcome was in-hospital death. Multivariable logistic regression models were adjusted for demographics, CVD risk factors, and CVD. Interaction between history of cancer with concomitant CVD and CVD risk factors were tested.Results: Among 8222 patients, 892 (10.8%) had a history of cancer and 1501 (18.3%) died. Cancer history had significant interaction with CVD risk factors of age, body mass index (BMI), and smoking history, but not underlying CVD itself. History of cancer was significantly associated with increased in-hospital death (among average age and BMI patients, adjusted odds ratio [aOR]=3.60, 95% confidence interval [CI]: 2.07-6.24; p<0.0001 in those with a smoking history and aOR=1.33, 95%CI: 1.01 - 1.76; p=0.04 in non-smokers). Among the cancer subgroup, prior use of chemotherapy within 2 weeks of admission was associated with in-hospital death (aOR=1.72, 95%CI: 1.05-2.80; p=0.03). Underlying CVD demonstrated a numerical but statistically nonsignificant trend toward increased mortality (aOR=1.18, 95% CI: 0.99 - 1.41; p=0.07).Conclusion: Among hospitalized COVID-19 patients, cancer history was a predictor of in-hospital mortality. Notably, among cancer patients, recent use of chemotherapy, but not underlying CVD itself, was associated with worse survival. These findings have important implications in cancer therapy considerations and vaccine distribution in cancer patients with and without underlying CVD and CVD risk factors.
Subject(s)
COVID-19ABSTRACT
BACKGROUND: Anxiety and stress like mental illnesses are the common outcomes of viral epidemics and pandemics. Novel coronavirus disease 2019 (COVID-19) outbreak caused by the severe acute respiratory syndrome coronavirus virus 2 (SARS-CoV-2) was first reported in Wuhan, China, and then spread all over the world in a short time. OBJECTIVES: To highlight and discuss the impact of COVID-19 pandemic on mental or psychological health. METHOD: Literature search and collection of the information were performed using PubMed, the reports from the World health organization, and the Center for disease control and prevention. RESULTS: COVID-19 infection has already been declared as a global pandemic, which in association with infodemic has increased the risk of psychiatric/psychological disorders. A large population of the world is prone to develop anxiety, depressive disorders, and other mental abnormalities. Therefore, timely psychological interventions and preventive strategies are required. Moreover, the infection has been reported to be linked with cerebrovascular conditions; therefore, patients with underlying cerebrovascular diseases should be given attention. CONCLUSION: COVID-19-mediated mental health complications and cerebrovascular conditions may cause a huge burden on healthcare communities in the future. Therefore, timely intervention and the development or application of preventive strategies are required to decrease the risk of neurological consequences.